Thermo Fisher Scientific GST Polyclonal Antibody, DyLight 549
DyLight 549 형광 표지된 GST 폴리클로날 항체로 Western blot, ICC/IF, ELISA 등에 적합. Schistosoma japonicum 유래 GST 전장 단백질을 면역원으로 사용. 염소 IgG 기반 항체로 높은 특이성과 신뢰성 제공. 연구용으로 단백질 상호작용 분석에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Full-length GST isolated from Schistosoma japonicum |
| Conjugate | DyLight™ 549 |
| Excitation/Emission Max | 556 / 570 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if solution is not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
Assay Information:
Immunoelectrophoresis shows a single precipitin arc against anti-Goat Serum and purified or partially purified Glutathione-S-Transferase (GST).
Target Information
GST (Glutathione S-transferase) is a 26 kDa protein found in both eukaryotes and prokaryotes, catalyzing various reactions. It is commonly used in the GST gene fusion system, allowing the purification of GST-fusion proteins via their high affinity for glutathione. These fusion proteins serve as important tools for studying direct protein–protein interactions. The GST gene fusion system is widely used in numerous expression vectors.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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