Thermo Fisher Scientific GFP Polyclonal Antibody, DyLight 488
DyLight 488 형광 표지된 GFP 폴리클로날 항체로, Western blot, ICC/IF, ELISA 등 다양한 응용에 적합. Goat IgG 기반으로 높은 특이성과 형광 안정성 제공. GFP 태그 단백질 검출 및 위치 추적 연구에 활용 가능.
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Thermo Fisher Scientific GFP Polyclonal Antibody, DyLight 488
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | 1:20,000–1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Green Fluorescent Protein (GFP) fusion protein, full-length (246aa) from Aequorea victoria |
| Conjugate | DyLight™ 488 |
| Excitation / Emission Max | 492 / 519 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if solution is not clear after standing.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified GFP (Aequorea victoria).
No cross-reactivity observed with Human, Mouse, or Rat serum proteins.
Target Information
The jellyfish Aequorea victoria produces Green Fluorescent Protein (GFP), which emits green light in bioluminescence reactions. GFP is widely used:
- As a reporter for gene expression in eukaryotic and prokaryotic systems
- As a protein tag for localization and movement studies
- In FRET and yeast two-hybrid assays for protein-protein interaction studies
GFP is a 27 kDa monomeric protein that forms its fluorescent pigment autocatalytically.
It absorbs blue light (395 nm) and emits green light (508 nm).
Its fluorescence is stable, species-independent, and suitable for diverse applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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