Thermo Fisher Scientific IFN gamma Monoclonal Antibody (XMG1.2), Brilliant Ultra Violet 805, eBioscience

Thermo Fisher Scientific IFN gamma Monoclonal Antibody (XMG1.2), Brilliant Ultra Violet 805, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: ≤ 0.25 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 615, 661, 737
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 660
• FITC
• Functional Grade
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The XMG1.2 antibody reacts specifically with mouse interferon (IFN) gamma, a 20 kDa cytokine produced by activated T, B, and NK cells. It is a neutralizing antibody that inhibits proliferation of normal and transformed cells in synergy with TNF alpha.
Reported for use in intracellular staining followed by flow cytometric analysis. Tested using stimulated mouse splenocytes and Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Recommended usage ≤ 0.25 µg per test (100 µL final volume).

Brilliant Ultra Violet™ 805 (BUV805) emits at 797 nm and is intended for cytometers with an ultraviolet (355 nm) laser. Use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409-75) to minimize non-specific polymer interactions.

Light Sensitivity: Protect vial and samples from light.
Fixation: Samples may be stored up to 3 days at 4°C in IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333).
Methanol Compatibility: Compatible with short-term methanol fixation (<1 hour).
Excitation: 355 nm; Emission: 797 nm; Laser: Ultraviolet.

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor produced by T-lymphocytes and NK cells. It is a glycoprotein homodimer (~45 kDa) with antiviral, antiproliferative, and immunomodulatory functions. Human IFN gamma does not cross-react with mouse IFN gamma. It regulates cytokine expression and activates the JAK-STAT pathway via IFN gamma receptor I and II. Mutations in the IFN gamma gene are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지