Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), Brilliant Ultra Violet 805, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 615, 661, 737
• Brilliant Violet 650
• eFluor 450, 506, 660
• FITC
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

Description:
The 4S.B3 monoclonal antibody reacts with interferon-gamma (IFN-γ), a 17 kDa cytokine produced by activated T and NK cells. IFN-γ exhibits antiviral and antiparasitic activity and modulates immune responses by interacting with high-affinity IFN-γ receptors on various cell types. Glycosylation does not affect its biological activity.

Applications Reported:
Suitable for intracellular staining followed by flow cytometric analysis.

Applications Tested:
Prediluted and validated for intracellular staining using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00). Recommended use at 5 µL (0.5 µg) per test in 100 µL final volume. Cell numbers can range from 10⁵–10⁸ cells/test.

Fluorochrome Information:
Brilliant Ultra Violet™ 805 (BUV805) is a tandem dye emitting at 797 nm, designed for UV (355 nm) laser-equipped cytometers.
Use Super Bright Complete Staining Buffer (Product #SB-4401) or Brilliant Stain Buffer (Product #00-4409-75) to minimize non-specific polymer interactions.

Light Sensitivity:
Protect vial and stained samples from light to avoid photo-induced oxidation.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333).
BUV805 is compatible with short-term methanol-based fixation (<1 hour).

Excitation / Emission / Laser:

• Excitation: 355 nm
• Emission: 797 nm
• Laser: Ultraviolet

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

IFN gamma (Interferon gamma, Type II interferon):
A macrophage activation factor produced by T-lymphocytes and NK cells in response to antigens and cytokines.
It functions as a homodimer (~45 kDa) and induces antiviral, antiproliferative, and immunomodulatory effects, including MHC induction and macrophage activation.
Human IFN-γ is species-specific and does not cross-react with mouse IFN-γ.
Mutations in the IFN-γ gene are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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