Thermo Fisher Scientific GPR68 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

5-10 µg/mL

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Bat, Horse, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic 17 amino acid peptide from N-terminus of human GPR68. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GPR68,uniProtId:Q15743-1,ncbiNodeId:9606,antigenRange:1,antigenLength:365,antigenImageFileName:PA5-32837_GPR68_Q15743-1_Rabbit.svg,antigenImageFileNamePDP:PA5-32837_GPR68_Q15743-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2550299

Product Specific Information

Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Bat, Elephant, Horse (100%); Marmoset, Mouse, Rat, Dog, Hamster, Rabbit, Pig (94%); Bovine, Panda (88%); Platypus (82%).

For IHC(P), use heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Target Information

GPR68 (also known as OGR1 - ovarian cancer G-protein coupled receptor 1) is a proton-sensing receptor involved in pH homeostasis. It represents an osteoblastic pH sensor regulating the cell-mediated response to acidosis in bone. GPR68 mediates its action by association with G proteins that stimulate inositol phosphate (IP) production or CA2+ mobilization. The receptor is almost silent at pH 7.8 but fully activated at pH 6.8. Also found to serve as a tumor suppressor in prostate cancer. Mutations to the gene result in amelogenesis imperfecta, hypomaturation type 2A6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.