Thermo Fisher Scientific GPR68 Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) [IHC (P)]

• Tested Dilution: 5–10 µg/mL

ELISA

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

Percent identity with other species by BLAST analysis:

• 100%: Human, Gorilla, Gibbon, Monkey, Bat, Elephant, Horse
• 94%: Marmoset, Mouse, Rat, Dog, Hamster, Rabbit, Pig
• 88%: Bovine, Panda
• 82%: Platypus

For IHC(P), use heat-induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Target Information

GPR68 (also known as OGR1 - ovarian cancer G-protein coupled receptor 1) is a proton-sensing receptor involved in pH homeostasis. It acts as an osteoblastic pH sensor regulating the cell-mediated response to acidosis in bone. GPR68 mediates its action via G proteins that stimulate inositol phosphate (IP) production or Ca²⁺ mobilization. The receptor is almost silent at pH 7.8 but fully activated at pH 6.8. It also functions as a tumor suppressor in prostate cancer. Mutations in this gene are associated with amelogenesis imperfecta, hypomaturation type 2A6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.