Thermo Fisher Scientific GPR77 Polyclonal Antibody
Rabbit polyclonal antibody targeting human GPR77 (C5AR2). Validated for IHC(P) and ELISA. High specificity via antigen affinity purification. Supplied in PBS with sodium azide, 1 mg/mL concentration. For research use only.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 10 µg/mL | Use heat-induced antigen retrieval in pH 6.0 citrate buffer. After primary incubation, use biotinylated secondary antibody followed by alkaline phosphatase–streptavidin and chromogen. |
| ELISA | Assay-dependent | — |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic 20 amino acid peptide from N-terminal extracellular domain of human C5L2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4 °C for short term. For long term, store at −20 °C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2550302 |
Product Specific Information
Percent identity with other species by BLAST analysis:
- Human (100%)
- Gorilla, Gibbon (95%)
- Monkey (85%)
- Marmoset (80%)
For IHC(P), use heat-induced antigen retrieval in citrate buffer (pH 6.0). After incubation with the primary antibody, slides should be incubated with biotinylated secondary antibody, followed by alkaline phosphatase–streptavidin and chromogen.
Target Information
C5AR2 (GPR77) is an orphan member of the anaphylatoxin receptor family. It is a seven-transmembrane G‑protein‑coupled receptor with an N‑terminal N‑glycosylation site, conserved cysteines, and multiple potential C‑terminal phosphorylation sites. It binds C5a and C5a‑des‑Arg, functioning as a regulated scavenger receptor that negatively modulates C5aR‑mediated inflammation. Northern blot analysis shows co‑expression with C5aR in various tissues and cells including neutrophils/macrophages, mast cells, dendritic cells, brain, lung, heart, kidney, liver, ovary, and testis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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