Thermo Fisher Scientific GPR77 Polyclonal Antibody
GPR77 단백질을 표적하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. IHC(P)와 ELISA에 사용 가능하며, 항원 친화 크로마토그래피로 정제됨. 인간과 높은 반응성, 안정적인 액상 형태로 연구용 적합.
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Applications
Immunohistochemistry (Paraffin) [IHC (P)]
- Tested Dilution: 10 µg/mL
ELISA
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic 20 amino acid peptide from C-terminal cytoplasmic domain of human C5L2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2550301 |
Product Specific Information
- Percent identity with other species by BLAST analysis: Human (100%), Gorilla (95%), Monkey (90%), Marmoset (85%)
- For IHC(P), use heat-induced antigen retrieval in pH 6.0 citrate buffer.
- After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Target Information
C5AR2 is an orphan member of the anaphylatoxin receptor family. It is a seven-transmembrane G-protein-coupled receptor with an N-terminal N-glycosylation site, conserved cysteines, and potential C-terminal phosphorylation sites.
It binds to C5a and C5a-des-Arg and acts as a regulated scavenger receptor with a negative modulatory influence on C5aR-mediated inflammation.
Northern blot analysis indicates co-expression with C5aR in tissues including neutrophils/macrophages, mast cells, dendritic cells, brain, lung, heart, kidney, liver, ovary, and testis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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