Thermo Fisher Scientific GPR68 Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 10–20 µg/mL
• 참고: Heat-induced antigen retrieval in pH 6.0 citrate buffer 권장

ELISA

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

Percent identity with other species by BLAST analysis:

• 100%: Human, Gorilla, Gibbon, Monkey, Marmoset, Rat
• 94%: Mouse, Bat, Dog, Hamster, Panda, Horse, Pig
• 89%: Bovine, Rabbit, Platypus
• 83%: Elephant

For IHC(P), use heat-induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Target Information

GPR68 (OGR1 - ovarian cancer G-protein coupled receptor 1)

• Proton-sensing receptor involved in pH homeostasis
• Acts as an osteoblastic pH sensor regulating cell-mediated response to acidosis in bone
• Mediates its action via G proteins that stimulate inositol phosphate (IP) production or Ca²⁺ mobilization
• Silent at pH 7.8, fully activated at pH 6.8
• Functions as a tumor suppressor in prostate cancer
• Gene mutations cause amelogenesis imperfecta, hypomaturation type 2A6

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.