Thermo Fisher Scientific MEK2 Monoclonal Antibody (GT3522)
MEK2 단백질을 인식하는 Mouse monoclonal antibody (Clone GT3522)로 Western blot 및 Immunocytochemistry에 사용 가능. Human에 반응하며, 예측 반응종은 Mouse, Rat, Dog, Pig 등. Protein G 정제, 1 mg/mL 농도, PBS buffer에 보존. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:3,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT3522 |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MEK2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787140 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (91%), Rat (92%), Dog (93%), Pig (93%), Chicken (91%), Bovine (93%).
- Positive Control: 293T, A431, HeLa, HepG2.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
MAP2K2 (MEK2) is a dual specificity serine/threonine kinase that plays a critical role in mitogen growth factor signal transduction.
Mitogen activated protein kinase 2 (MEK2 or MAPKK2) belongs to a family of tyrosine/threonine protein kinases that activate ERK1/2 and MAPK enzymes by phosphorylating residues within the TEY motif in the activation loop.
MEK1 and MEK2 are activated by dual phosphorylation on serine 218 and 222 in the activation loop.
Threonine 292 of MEK1 is phosphorylated by ERK2, providing a negative feedback regulation mechanism by suppressing MEK1 activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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