Thermo Fisher Scientific MEK2 Monoclonal Antibody (GT3522)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

GT3522

Immunogen

Recombinant protein encompassing a sequence within the center region of human MEK2.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2787140

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (91%), Rat (92%), Dog (93%), Pig (93%), Chicken (91%), Bovine (93%).

Positive Control: 293T, A431, HeLa, HepG2.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

MAP2K2 (MEK2) is a dual specificity serine/threonine kinase that is known to play a critical role in mitogen growth factor signal transduction. Mitogen activated protein kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.