Thermo Fisher Scientific H4K20me2 Monoclonal Antibody (GT1851)
H4K20me2 단백질을 인식하는 Mouse monoclonal 항체로 Western blot, ICC/IF, IP, ChIP, Dot blot 등에 사용 가능. Human에 반응하며 HeLa 세포를 Positive control로 사용. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
| ChIP assay (ChIP) | Assay-dependent |
| Dot blot (DB) | 1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT1851 |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H4K20me2 (dimethyl Lys20) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787142 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Xenopus laevis (100%).
- Positive Control: HeLa.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped to form nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and contributes to chromatin compaction into higher-order structures. This gene is intronless, encodes a member of the histone H4 family, and is located within the large histone gene cluster on chromosome 6. Transcripts lack polyA tails and contain a palindromic termination element.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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