
Thermo Fisher Scientific CD53 Chimeric Recombinant Rabbit Monoclonal Antibody (HD77)
CD53 단백질을 표적하는 재조합 토끼 단클론 항체로, 인간 시료에 반응합니다. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합합니다. 고순도 Protein A 정제, PBS 버퍼, ProClin 300 포함. 연구용으로만 사용 가능합니다.
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Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | HD77 |
| Immunogen | This antibody was raised by immunising mice with human leukemia cells. Target: CD53 (UniProt ID: P19397-1), Antigen Range: 1–219 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074688 |
Product Specific Information
Specificity: This antibody is specific for the extracytoplasmic part of human CD53, a pan-leukocyte antigen.
Target Information
CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. It forms complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81, and CD82, modulating various signaling processes. CD53 is involved in radioresistancy of tumor cells and has anti-apoptotic effects. It is up-regulated during T cell development and macrophage activation, and down-regulated upon neutrophil stimulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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