Thermo Fisher Scientific CD53 Recombinant Mouse Monoclonal Antibody (HD77)
Recombinant mouse monoclonal antibody targeting human CD53 (HD77). Suitable for WB, IHC, ICC/IF, Flow, and IP. Specific to extracytoplasmic region of CD53. High purity Protein A preparation, 1 mg/mL in PBS with ProClin 300. For research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | HD77 |
| Immunogen | This antibody was raised by immunising mice with human leukemia cells. Target: CD53 (UniProt ID: P19397-1), Human (NCBI Node ID: 9606), Antigen Range: 1–219, Antigen Length: 219 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074687 |
Product Specific Information
Specificity: This antibody is specific for the extracytoplasmic part of human CD53, a pan-leukocyte antigen.
Target Information
CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. It forms complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4, and tetraspanins CD37, CD81, CD82, modulating various signaling processes. CD53 contributes to radioresistance and has anti-apoptotic effects. In thymus, CD53 is up-regulated during T cell development and strongly expressed in macrophages exposed to bacterial lipopolysaccharide, while neutrophil stimulation down-regulates its expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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