Thermo Fisher Scientific GARP Monoclonal Antibody (YGIC86), PE, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test
• Publications: View 11 publications

Product Specifications

Additional Available Formats

• APC
• eFluor 450
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The YGIC86 monoclonal antibody reacts with mouse Glycoprotein A Repetitions Predominant (GARP, also known as Lrrc32 or Garpin), an ~80 kDa glycoprotein expressed on the cell surface. GARP expression has been reported in placenta, lung, kidney, heart, ovary, liver, skeletal muscle, and pancreas. It is also observed on megakaryocytes, platelets, and activated regulatory T (Treg) cells.

Expression of GARP on Treg cells is essential for their suppressive function, possibly related to its role as a receptor for LAP/TGF-β.
For platelet staining, use the Foxp3 Staining Buffer Set (Product #00-5523) before staining with YGIC86. Fixation/permeabilization is not required for Treg cell staining.

• Cross-reactivity: Does not cross-react with rat GARP.
• Applications Reported: Flow cytometric analysis.
• Applications Tested: Validated for flow cytometry using stimulated mouse spleen cells at ≤0.25 µg/test (100 µL final volume).
• Recommended Laser: Blue, Green, or Yellow-Green Laser.
• Excitation/Emission: 488–561 nm / 578 nm.
• Filtration: 0.2 µm post-manufacturing filtered.

Target Information

GARP (leucine rich repeat containing 32) is a type I membrane protein with twenty-two leucine-rich repeats and a short intracytoplasmic region. It was identified from gene expression profiling of Treg and Th cells following TCR stimulation. Since GARP is expressed in CD4+, CD25+, FoxP3+ Treg cells, it serves as a potential surface marker for these cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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