Thermo Fisher Scientific HELIOS Monoclonal Antibody (22F6), PE, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.03 µg)/test
• Publications: View 20 publications

Product Specifications

Available Conjugate Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 786
• eFluor 450
• FITC
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
This 22F6 monoclonal antibody reacts with human, mouse, and canine Helios. Helios is a zinc-finger transcription factor similar to Ikaros, expressed in hematopoietic stem cells in both embryo and adult mice. The highest expression is found in regulatory T cells, while it is not expressed in mature B cells, dendritic cells, or myeloid cells. Helios can form homodimers or heterodimers with other Ikaros family members to regulate transcription. Eight isoforms exist, some with dominant-negative functions, upregulated in lymphoid malignancies such as T-acute lymphoblastic leukemia.

22F6 has been reported to cross-react with rat Helios.

Applications Reported:
Intracellular staining followed by flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by intracellular staining and flow cytometry using mouse lymph node cells and the Foxp3/Transcription Factor Buffer Set (Product #00-5523-00).
Refer to BestProtocols® - Staining intracellular Antigens for Flow Cytometry (Protocol B: One-step protocol for intracellular (nuclear) proteins).

• Recommended usage: 5 µL (0.03 µg)/test
• Excitation: 488–561 nm
• Emission: 578 nm
• Compatible Lasers: Blue, Green, Yellow-Green Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

This gene encodes a member of the Ikaros family of zinc-finger proteins. The family members (Ikaros, Aiolos, Helios) are hematopoietic-specific transcription factors regulating lymphocyte development. Helios forms homo- or heterodimers with other Ikaros family members and functions mainly in early hematopoietic development. Multiple transcript variants exist, though the biological significance of some remains uncertain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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