Thermo Fisher Scientific HELIOS Monoclonal Antibody (22F6), PE, eBioscience
Helios 단백질을 인식하는 22F6 클론의 단클론 항체로, PE 형광표지되어 세포내 염색 및 유세포분석에 적합합니다. 사람, 마우스, 개 시료에 반응하며, 조절 T세포 연구에 활용됩니다. 4°C 보관, 동결 금지.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.03 µg)/test
- Publications: View 20 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 22F6 |
| Conjugate | PE |
| Excitation/Emission Max | 565/576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572758 |
Available Conjugate Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Brilliant UV 395
- Brilliant UV 563
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 786
- eFluor 450
- FITC
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
Description:
This 22F6 monoclonal antibody reacts with human, mouse, and canine Helios. Helios is a zinc-finger transcription factor similar to Ikaros, expressed in hematopoietic stem cells in both embryo and adult mice. The highest expression is found in regulatory T cells, while it is not expressed in mature B cells, dendritic cells, or myeloid cells. Helios can form homodimers or heterodimers with other Ikaros family members to regulate transcription. Eight isoforms exist, some with dominant-negative functions, upregulated in lymphoid malignancies such as T-acute lymphoblastic leukemia.
22F6 has been reported to cross-react with rat Helios.
Applications Reported:
Intracellular staining followed by flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by intracellular staining and flow cytometry using mouse lymph node cells and the Foxp3/Transcription Factor Buffer Set (Product #00-5523-00).
Refer to BestProtocols® - Staining intracellular Antigens for Flow Cytometry (Protocol B: One-step protocol for intracellular (nuclear) proteins).
- Recommended usage: 5 µL (0.03 µg)/test
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
This gene encodes a member of the Ikaros family of zinc-finger proteins. The family members (Ikaros, Aiolos, Helios) are hematopoietic-specific transcription factors regulating lymphocyte development. Helios forms homo- or heterodimers with other Ikaros family members and functions mainly in early hematopoietic development. Multiple transcript variants exist, though the biological significance of some remains uncertain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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