Thermo Fisher Scientific CD371 (Clec12A) Monoclonal Antibody (HB3), PE, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

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Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HB3

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11043413

Product Specific Information

Description: This HB3 monoclonal antibody reacts with human CD371, which is also known as Clec12A or Myeloid Inhibitory C-type Lectin-like receptor (MICL). CD371 is a highly glycosylated transmembrane receptor with a cytoplasmic ITIM that associates with SHP1 and SHP2. CD371 is expressed mainly on granulocytes, monocytes, macrophages, and dendritic cells, including plasmacytoid dendritic cells. Expression of CD371 on NK cells remains controversial. Although the function of CD371 is not fully understood, studies suggest that this receptor plays a role in inflammation and LPS activation.

Applications Reported: This HB3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HB3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD371 is also known as Clec12A or Myeloid Inhibitory C-type Lectin-like receptor (MICL). CD371 is a highly glycosylated transmembrane receptor with a cytoplasmic ITIM that associates with SHP1 and SHP2. CD371 is expressed mainly on granulocytes, monocytes, macrophages, and dendritic cells, including plasmacytoid dendritic cells. Expression of CD371 on NK cells remains controversial. Although the function of CD371 is not fully understood, studies suggest that this receptor plays a role in inflammation and LPS activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.