Thermo Fisher Scientific ITGA2 Monoclonal Antibody (16B4)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunohistochemistry (Frozen) (IHC (F))

30-40 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Flow Cytometry (Flow)

30 µg/mL

-

ELISA (ELISA)

10 µg/mL

-

Immunoprecipitation (IP)

25-50 µg/mL

-

Functional Assay (Functional)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

16B4

Immunogen

Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD49b (Integrin alpha 2),uniProtId:P17301-1,ncbiNodeId:9606,antigenRange:1-1181,antigenLength:1181,antigenImageFileName:MA1-80784_CD49b_Integrin_alpha_2_P17301-1_House_mouse.svg,antigenImageFileNamePDP:MA1-80784_CD49b_Integrin_alpha_2_P17301-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_928516

Product Specific Information

This antibody does not react with rat.

Sodium azide removal by dialysis is recommended prior to use in functional assays. Heat-mediated antigen retrieval using Tris/EDTA (pH 9) is recommended for the staining of paraffin sections.

Mouse anti Human CD49b antibody, clone 16B4 recognizes human CD49b, also known as integrin α2, collagen receptor, Platelet membrane glycoprotein Ia or VLA-2 subunit alpha. CD49b is a 1181 amino acid approximately 160 kDa single pass type-1 transmembrane glycoprotein possessing multiple FG-GAP repeats and a single VWFA domain.

Target Information

CD49b (Integrin alpha 2, Integrin alpha 2 chain, VLA-2 alpha chain, HM alpha 2) is a member of the integrin family. It is a glycoprotein with molecular weight of 150 kD, and it complexes with CD29 (Integrin beta 1) to form the heterodimeric integrin VLA-2 (integrin alpha 2 beta 1, or GPIa/IIa) complex. VLA-2 is an extracellular receptor for laminin, collagen, and fibronectin, and interaction with its ligands results in the activation of intracellular signaling pathways. It has reported roles in VEGF-induced angiogenesis in vivo, as well as adhesion and lymphocyte activation. CD49b is expressed by NK cells, NK-T cells, monocytes, platelets, and epithelial cells. It is also expressed on adaptive immune cells such as T and B cells, specifically on a subset of CD4+ T cells in the spleen, on intraepithelial and lamina propria lymphocytes in the intestine, as well as on a population of peripheral CD4+ type 1 T regulatory (Tr1) cells that co-express LAG-3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.