Thermo Fisher Scientific EpCAM/CD326 Recombinant Rabbit Monoclonal Antibody (EGP40, 4546R)
EpCAM/CD326에 특이적인 재조합 토끼 단클론 항체로, 인체 시료의 면역조직화학(IHC) 분석에 적합합니다. HEK293 세포에서 발현되었으며, Protein A/G로 정제된 액상 형태입니다. Ep-CAM 관련 종양 연구 및 세포 접착 분자 분석에 활용됩니다.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | EGP40, 4546R |
| Immunogen | Recombinant full-length human Ep-CAM protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C (preferred) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a Ca²⁺-independent homotypic adhesion molecule and influences cell cycle, proliferation, and metabolism by inducing proto-oncogene c-myc and cell cycle regulators cyclin A and E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression of Ep-CAM is linked to enhanced epithelial proliferation and is a marker for tumors of epithelial lineage. It is expressed on the baso-lateral surface of most simple epithelia and many carcinoma types, and helps distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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