Thermo Fisher Scientific Ep-CAM/CD326 Recombinant Rabbit Monoclonal Antibody (EGP40, 2041R)
Ep-CAM/CD326 단백질을 인식하는 재조합 토끼 모노클로날 항체로, Western blot 및 면역조직화학(IHC)에 적합합니다. 인간, 개, 고양이 반응성을 가지며 HEK293 세포에서 발현되었습니다. 고순도 Protein A 정제, 액상 형태로 제공되며 연구용으로 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Cat, Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | EGP40, 2041R |
| Immunogen | Recombinant human EpCAM fragment from extracellular domain (around aa100–224) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.
Target Information
Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial cells.
It acts as a calcium-independent homotypic adhesion molecule and influences cell cycle, proliferation, and metabolism by inducing proto-oncogene c-myc and cyclins A and E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth.
Overexpression is linked to enhanced epithelial proliferation and is a marker for carcinomas of epithelial origin.
Ep-CAM is expressed on the basolateral surface of most simple epithelia and many carcinoma types, and can distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일: 4072-RBM21-P1ABX_EpCAM_CD326_P16422-1_Rabbit.svg / 4072-RBM21-P1ABX_EpCAM_CD326_P16422-1_Rabbit_PDP.jpeg)
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