Thermo Fisher Scientific CD244 Monoclonal Antibody (eBioC1.7 (C1.7)), NovaFluor Red 685, eBioscience

Thermo Fisher Scientific CD244 Monoclonal Antibody (eBioC1.7 (C1.7)), NovaFluor Red 685

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.4 µg)/test

Product Specifications

Additional Formats

• Biotin
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• PE
• PE-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The eBioC1.7 monoclonal antibody reacts with human CD244 (2B4, p38). CD244 is a 38 kDa protein expressed on NK cells, subsets of CD8+ T cells, γδ T cells, monocytes, basophils, and eosinophils. Binding of CD48 to CD244 results in NK cell activation and enhanced cytotoxicity and IFN-γ secretion.

Binding of the C1.7 monoclonal antibody to CD244 induces tyrosine phosphorylation and recruitment of SAP (SLAM-associated protein). In X-linked lymphoproliferative disorder (XLPD), SAP mutation prevents binding to phosphorylated CD244.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells.
Use at 5 µL (0.4 µg/mL) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

NovaFluor Dyes Compatibility

• Not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Recommended: Invitrogen LIVE/DEAD Fixable Dead Cell stains.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01)

• Improved formulation over previous CellBlox buffer.
• Required for optimal staining with NovaFluor conjugates.
• Add 5 µL per sample to antibody cocktails prior to combining with cells.
• For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor Conjugate Technology

• Based on Phiton™ technology using nucleic acid dye structures for engineered fluorescent signatures minimizing spillover and spread.
• Excitation: 637 nm; Emission: 685 nm; Laser: 633–640 nm (Red).

Target Information

CD244 (2B4, SLAMF4) is a cell surface receptor expressed on NK cells and some T cells, mediating non-MHC restricted killing. Interaction with SLAMF2 modulates NK-cell cytolytic activity.
Altered CD244 expression is associated with autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis, and lymphoproliferative syndrome.
Alternative splicing generates multiple isoforms of CD244.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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