Thermo Fisher Scientific CXCL13 Polyclonal Antibody, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

AA Sequence of Recombinant Protein:
VLEVYYTSLR CRCVQESSVF IPRRFIDRIQ ILPRGNGCPR KEIIVWKKNK SIVCVDPQAE WIQRMMEVLR KRSSSTLPVP VFKRKIP

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human BCA-1 (CXCL13). Anti-Human BCA-1 (CXCL13)-specific antibody was purified by affinity chromatography employing an immobilized Human BCA-1 (CXCL13) matrix.

Sandwich ELISA:
To detect Human BCA-1 (CXCL13) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human BCA-1 (CXCL13) (500-P141Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human BCA-1 (CXCL13).

Western Blot:
To detect Human BCA-1 (CXCL13) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human BCA-1 (CXCL13) is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

CXCL13, also known as BCA-1 and Angie, is a small chemokine strongly expressed in secondary lymphoid organs. It promotes migration of B lymphocytes (both B-1 and B-2) to follicles of the spleen, lymph nodes, and Peyer’s patches. It is produced by macrophages; in mice, large peritoneal macrophages, Kupffer cells, and lung macrophages (both CD11c+ and CD11c−) are major producers.

The cellular ligand for CXCL13 is CXCR5 (also known as BLR-1), expressed on B cells, Tfh cells, and some memory T cells. CXCL13 plays an important role in the development of chronic lymphocytic leukemia (CCL) and serves as a plasma biomarker for germinal center activity and generation of broadly neutralizing antibodies against HIV.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.