Thermo Fisher Scientific CXCL13 Polyclonal Antibody, PeproTech
CXCL13 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, ELISA, Immunostaining에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, Human CXCL13 검출에 높은 특이성과 민감도를 제공합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.5–2.0 µg/mL | - |
| Immunostaining (IS) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human BCA-1 (CXCL13) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929453 |
Product Specific Information
AA Sequence of Recombinant Protein:
VLEVYYTSLR CRCVQESSVF IPRRFIDRIQ ILPRGNGCPR KEIIVWKKNK SIVCVDPQAE WIQRMMEVLR KRSSSTLPVP VFKRKIP
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human BCA-1 (CXCL13). Anti-Human BCA-1 (CXCL13)-specific antibody was purified by affinity chromatography employing an immobilized Human BCA-1 (CXCL13) matrix.
Sandwich ELISA:
To detect Human BCA-1 (CXCL13) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human BCA-1 (CXCL13) (500-P141Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human BCA-1 (CXCL13).
Western Blot:
To detect Human BCA-1 (CXCL13) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human BCA-1 (CXCL13) is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
CXCL13, also known as BCA-1 and Angie, is a small chemokine strongly expressed in secondary lymphoid organs. It promotes migration of B lymphocytes (both B-1 and B-2) to follicles of the spleen, lymph nodes, and Peyer’s patches. It is produced by macrophages; in mice, large peritoneal macrophages, Kupffer cells, and lung macrophages (both CD11c+ and CD11c−) are major producers.
The cellular ligand for CXCL13 is CXCR5 (also known as BLR-1), expressed on B cells, Tfh cells, and some memory T cells. CXCL13 plays an important role in the development of chronic lymphocytic leukemia (CCL) and serves as a plasma biomarker for germinal center activity and generation of broadly neutralizing antibodies against HIV.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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