Thermo Fisher Scientific MIP-3 alpha Polyclonal Antibody
인간 MIP-3α(CCL20)에 특이적인 토끼 폴리클로날 항체로, Western blot 및 ELISA에 적합합니다. 단백질 A로 정제된 비결합 항체이며, 동결건조 형태로 제공됩니다. 연구용으로만 사용 가능하며, 장기 보관 시 -20°C 이하에서 보관을 권장합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1:20,000–1:100,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-3α protein (CCL20, UniProt ID: P78556-1, antigen range: 1–96). |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to reconstitution.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only immediately prior to use.
In ELISA and other immunoreactive assays, this antibody recognizes both native and recombinant human MIP-3α in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Target Information
Macrophage Inflammatory Protein-3 alpha (MIP-3α), also known as CCL20, is expressed in the liver, lungs, lymph nodes, and peripheral blood lymphocytes. MIP-3α is strongly upregulated by inflammatory signals and downregulated by the anti-inflammatory cytokine IL-10. It signals through the G protein-coupled receptor CCR6 and acts as a chemoattractant for lymphocytes and dendritic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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