Thermo Fisher Scientific MCP-1 Polyclonal Antibody
Human MCP-1 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western Blot 및 ELISA에 적합합니다. 고순도의 이온교환 크로마토그래피로 정제되었으며, 동물 간 교차 반응 가능성이 있습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with mature length recombinant human MCP-1 protein produced in E. coli. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific for human MCP-1 protein. A BLAST analysis indicates cross-reactivity with MCP-1 from human sources (100% homology with the immunizing sequence). Based on 98–100% homology, cross-reactivity may occur with MCP-1 from chimpanzee, orangutan, macaque, and baboon. Cross-reactivity with MCP-1 from other sources has not been determined.
Target Information
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are secreted proteins involved in immunoregulatory and inflammatory processes. The encoded protein is structurally related to the CXC subfamily of cytokines, characterized by two cysteines separated by a single amino acid.
This cytokine shows chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It is implicated in diseases with monocytic infiltrates such as psoriasis, rheumatoid arthritis, and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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