Thermo Fisher Scientific H4K20me1 Monoclonal Antibody (5E10-D8), ChIP-Verified

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

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Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Flow Cytometry (Flow)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

ChIP assay (ChIP)

1-5 µg

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Dot blot (DB)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5E10-D8

Immunogen

Synthetic peptide within Human Histone H4 (mono methyl K20)(Immunogen sequence: 15-AKRHR(Kme)VLRDN-25 (branched)) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:H4K20me1,uniProtId:P62805-1,ncbiNodeId:9606,antigenRange:15-25|16-20,antigenLength:103,antigenImageFileName:MA5-18067_H4K20me1_P62805-1_House_mouse.svg,antigenImageFileNamePDP:MA5-18067_H4K20me1_P62805-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539450

Product Specific Information

This antibody is specific for K20 mono-methylated histone H4 and does not cross-react with histone 3 or tri-, di-, or unmethylated histone H4. This antibody is predicted to react with Xenopus laevis, Arabidopsis thaliana, Fruit fly (Drosophila melanogast), and most mammals based on sequence homology.

This target has a predicted molecular weight of 11 kDa. Actual Western blot results show a band at 13 kDa. This can be blocked using mono-methyl K20 Histone H4 peptide.

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Target Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.