Thermo Fisher Scientific H4K20me1 Monoclonal Antibody (5E10-D8), ChIP-Verified
H4K20me1 단일메틸화 히스톤 H4에 특이적인 마우스 단클론 항체로, ChIP 검증 완료. WB, IHC, ICC, Flow 등 다양한 응용 가능. 교차반응 없음, 1 mg/mL 농도 액상 형태. 단기 4°C, 장기 -20°C 보관 권장.
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Thermo Fisher Scientific H4K20me1 Monoclonal Antibody (5E10-D8), ChIP-Verified
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
| Flow Cytometry (Flow) | Assay-dependent | - |
| ELISA | Assay-dependent | - |
| ChIP assay (ChIP) | 1–5 µg | View 1 publication |
| Dot blot (DB) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5E10-D8 |
| Immunogen | Synthetic peptide within Human Histone H4 (mono methyl K20). Sequence: 15-AKRHR(Kme)VLRDN-25 (branched) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539450 |
Product Specific Information
This antibody is specific for K20 mono-methylated histone H4 and does not cross-react with histone H3 or tri-, di-, or unmethylated histone H4.
It is predicted to react with Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, and most mammals based on sequence homology.
- Predicted molecular weight: 11 kDa
- Observed band in Western blot: 13 kDa
- Blocking peptide: mono-methyl K20 Histone H4 peptide
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Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fiber in eukaryotes.
Two molecules of each core histone (H2A, H2B, H3, H4) form an octamer around which ~146 bp of DNA is wrapped, forming nucleosomes.
Linker histone H1 interacts with linker DNA between nucleosomes, aiding chromatin compaction.
The histone H4 gene is intronless, lacks polyA tails, and contains a palindromic termination element. It is located in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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