Thermo Fisher Scientific PP2A B-alpha Monoclonal Antibody (2G9)
Western blot 및 Immunoprecipitation에 사용 가능한 PP2A B-alpha 단일클론 항체. Human, Mouse, Rat 반응성. Protein G 정제, 1 mg/mL 농도, PBS buffer에 0.1% sodium azide 포함. 4°C 단기, -20°C 장기 보관 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2G9 |
| Immunogen | Peptide from rat PP2A isoform alpha (398-CASGKRKKDEISVD-411) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539447 |
Product Specific Information
This antibody reacts very strongly with rat alpha and delta isoforms, and weakly with the human beta and rabbit gamma isoforms.
It is predicted to react with porcine, rabbit, chicken, and xenopus samples based on sequence homology.
Target Information
Gene: PPP2R2A (Protein Phosphatase 2 Regulatory Subunit Balpha)
Function: Protein coding gene involved in regulation of cell cycle and mitotic signaling.
Associated Diseases: Lung Cancer, Hepatocellular Carcinoma
Related Pathways: Cell Cycle (Mitotic), p70S6K Signaling
Molecular Function (GO): Protein serine/threonine phosphatase activity, Protein phosphatase regulator activity
Paralog: PPP2R2D
The B regulatory subunit modulates substrate selectivity and catalytic activity, directing the catalytic enzyme to specific subcellular compartments.
Essential for PP2A-mediated dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis and promoting the G2/M checkpoint.
Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, involved in negative control of cell growth and division.
This gene encodes the alpha isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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