Thermo Fisher Scientific Human TGFBR2, GST Tag Recombinant Protein
Human TGFBR2 recombinant protein with GST tag for kinase binding assays. Expressed in insect cells and validated for LanthaScreen Eu Kinase Binding Assay. Suitable for studying inhibitor binding and allosteric regulation. Stable at -80°C with purified ...
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Thermo Fisher Scientific Human TGFBR2, GST Tag Recombinant Protein
Applications
- Kinase Assay (KA)
Tested Dilution
- Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | Baculovirus |
| Amino Acid Sequence | 194–567 |
| Tag | GST-tag |
| Molecular Weight | 70.3 kDa |
| Class | Recombinant |
| Type | Protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Purified |
| Storage Buffer | 50 mM Tris HCl, pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.02% NP-40, 50% glycerol, 2 mM DTT |
| Contains | No preservative |
| Storage Conditions | -80°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Dry ice |
| Protein Form | Truncated |
Product Specific Information
For maximum recovery, please spin prior to use. Aliquots of 5 µg, 10 µg, and 20 µg sizes are not recommended; use in original packaging until exhausted. For larger sizes, aliquoting can reduce freeze/thaw cycles, but aliquots below 20 µL are not recommended. Do not store kinase diluted. Properly stored at -80°C, product is guaranteed for 6 months from purchase.
This recombinant fusion protein consists of the catalytic domain of transforming growth factor beta receptor 2 (TGFBR2) and a glutathione S-transferase (GST) tag. It is validated in the LanthaScreen™ Eu Kinase Binding Assay. No special activation was performed.
Key Features
- Compatible and validated with LanthaScreen™ Eu Kinase Binding Assay
- Expressed in insect cells
- GST-tagged for use with Eu-labeled GST antibody
Easily Screen for Kinase Binding
The LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay enabling measurement of inhibitor binding to non-activated kinases, simple off-rate measurements, and study of type III (allosteric) inhibitors.
The assay uses a mix-and-read format with one-hour incubation and no development steps. Binding of an Alexa Fluor™ tracer to a tagged kinase is detected via europium-labeled anti-GST antibody. Simultaneous binding produces strong FRET; displacement by a kinase inhibitor reduces FRET.
TGFBR2 for the LanthaScreen Kinase Binding Assay
This insect cell-expressed recombinant fusion protein of the TGFBR2 catalytic domain and GST tag has been validated with:
- Tracer 178 (Cat. No. PV5593)
- Eu-labeled GST Antibody (Cat. No. PV5594)
Related Link
Target Information
Transforming Growth Factor, beta receptor 2 (TGFBR2; also known as AAT3, FAA3, MFS2, RIIC, LDS1B, LDS2B, TAAD2, TGFR-2, TGFbeta-RII) is a member of the TGF-beta receptor serine/threonine kinase family involved in regulation of cell proliferation.
TGFBR2 encodes the gamma subunit of the catalytic core of mitochondrial ATP synthase, which catalyzes ATP synthesis using a proton gradient across the inner membrane during oxidative phosphorylation. The enzyme consists of F1 (soluble catalytic core) and Fo (membrane proton channel) complexes. Alternative splicing produces different isoforms, and a pseudogene exists on chromosome 14.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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