Thermo Fisher Scientific SF3a120/SAP114 Polyclonal Antibody
SF3a120/SAP114 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, IP 등 다양한 응용에 적합합니다. 인간 반응성, 항원 친화 크로마토그래피 정제, 액상 형태이며 4°C 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 500 and 550 of Human splicing factor 3a, subunit 1, 120kDa |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Mouse and Bovine
Target Information
This gene encodes subunit 1 of the splicing factor 3a (SF3A1) protein complex. The SF3A complex consists of subunits 1, 2, and 3, and is essential for converting 15S U2 snRNP into an active 17S particle involved in pre-mRNA splicing.
Subunit 1 belongs to the SURP protein family, characterized by SURP motifs mediating RNA binding. The amino-terminal half contains tandem SURP motifs, while the carboxy-terminal half includes a proline-rich region and a ubiquitin-like domain.
Binding studies show SURP motifs are necessary for interaction with subunit 3, with possible contacts to subunit 2 through carboxy-terminal sequences. Alternative splicing produces multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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