Thermo Fisher Scientific SF3a120/SAP114 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–5 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Based on 100% sequence identity, this antibody is predicted to react with Mouse and Bovine

Target Information

This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2, and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing.
Subunit 1 belongs to the SURP protein family, named for the SURP motifs that mediate RNA binding. It contains tandemly repeated SURP motifs in its amino-terminal half and a proline-rich region with a ubiquitin-like domain in the carboxy-terminal half.
Binding studies show that SURP motifs are required for interaction with subunit 3, while subunit 2 may bind through sequences carboxy-terminal to the SURP motifs.
Alternative splicing results in multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.