Thermo Fisher Scientific SF3a120/SAP114 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt.
• For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
• Based on 100% sequence identity, this antibody is predicted to react with Bovine.

Target Information

This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2, and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing.
Subunit 1 belongs to the SURP protein family, named for the SURP motifs that mediate RNA binding. It contains tandemly repeated SURP motifs in its amino-terminal half and a proline-rich region plus a ubiquitin-like domain in its carboxy-terminal half.
Binding studies show that SURP motifs are essential for binding to subunit 3, while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs.
Alternative splicing results in multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.