Thermo Fisher Scientific KDEL Monoclonal Antibody (2C1), FITC
KDEL 서열을 인식하는 FITC 결합 단클론 항체로, 인간, 마우스, 랫트 시료에서 ER 단백질 검출에 적합합니다. Western blot 및 파라핀 절편 IHC에 사용 가능하며, 단백질 G로 정제된 액상 형태로 제공됩니다. 4°C, 암소 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2C1 |
| Immunogen | KDEL peptide (C-AVKDEL-COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA) |
| Conjugate | FITC |
| Excitation/Emission Max | 498/517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932324 |
Available Formats
Product Specific Information
A 1:1,000 dilution of MA5-45870 was sufficient for detection of KDEL in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Detects KDEL-tagged proteins.
Target Information
The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins.
GRP78 (78 kDa) and GRP94 (94 kDa), as well as protein disulfide isomerase (PDI), share the C-terminal KDEL sequence.
This sequence is necessary for ER retention and sufficient to reduce secretion of proteins from the ER. The retention process is mediated by a KDEL receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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