Thermo Fisher Scientific KDEL Monoclonal Antibody (2C1), APC
KDEL 서열을 인식하는 Mouse monoclonal antibody로 APC 형광 표지되어 있음. Western blot과 IHC(Paraffin)에서 사용 가능. Human, Mouse, Rat 반응성. 단백질 ER 유지 연구에 적합. 4°C 보관, Protein G 정제.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:25
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2C1 |
| Immunogen | KDEL peptide (C-AVKDEL-COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA) |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932323 |
Additional Formats
- Unconjugated (MA5-27580)
- FITC (MA5-45870)
- PE (MA5-45872)
- PerCP (MA5-45871)
- Request custom conjugation
Product Specific Information
A 1:1,000 dilution of MA5-45869 was sufficient for detection of KDEL in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects KDEL tagged proteins.
Target Information
The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins.
GRP78 and GRP94 (78 and 94 kDa glucose regulated proteins) and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence.
The presence of carboxy-terminal KDEL is necessary for ER retention and sufficient to reduce secretion of proteins from the ER.
This retention is mediated by a KDEL receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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