Thermo Fisher Scientific KDEL Monoclonal Antibody (1F2), PE
KDEL 단백질을 검출하기 위한 PE 결합 마우스 모노클로날 항체. Western blot에 최적화되어 있으며 1:1,000 희석에서 높은 민감도 제공. ER 잔류 단백질 연구에 적합. 4°C 보관, 연구용 전용.
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Applications
- Western Blot (WB)
Tested Dilution
- 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1F2 |
| Immunogen | KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA) |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
A 1:1,000 dilution of MA5-45868 was sufficient for detection of KDEL in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects KDEL tagged proteins.
Target Information
The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins.
78 and 94 kDa glucose regulated proteins, GRP78 and GRP94 respectively, and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence.
The presence of carboxy-terminal KDEL appears to be necessary for ER retention and sufficient to reduce secretion of proteins from the ER.
This retention is reported to be mediated by a KDEL receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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