Thermo Fisher Scientific KDEL Monoclonal Antibody (2C1), PerCP
KDEL 서열을 인식하는 단클론 항체로, PerCP 형광 표지된 형태입니다. Western blot 및 IHC(P)에서 검증되었으며, 인간, 마우스, 랫트 시료에 반응합니다. 단백질 ER 보유 신호(KDEL) 검출에 적합하며, 고순도의 Protein G 정제 항체입니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:25
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2C1 |
| Immunogen | KDEL peptide (C-AVKDEL-COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA) |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932325 |
Additional Formats
Product Specific Information
A 1:1,000 dilution of MA5-45871 was sufficient for detection of KDEL in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects KDEL-tagged proteins.
Target Information
The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins.
78 and 94 kDa glucose-regulated proteins, GRP78 and GRP94 respectively, and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence.
The presence of carboxy-terminal KDEL appears to be necessary for ER retention and sufficient to reduce secretion of proteins from the ER.
This retention is mediated by a KDEL receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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