Thermo Fisher Scientific Claudin 4 Monoclonal Antibody (3E2C1)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 - 3 µg/mL

View 69 publications 69 publications

Immunohistochemistry (IHC)

2-3 µg/mL

View 35 publications 35 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 31 publications 31 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 10 publications 10 publications

Immunocytochemistry (ICC/IF)

-

View 50 publications 50 publications

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 23 publications 23 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Amphibian, Bovine, Dog, Fish, Human, Mouse, Non-human primate, Pig, Rabbit, Rat, Virus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3E2C1

Immunogen

Synthetic peptide corresponding to a 22 amino acid sequence derived from the C-terminal region of human Claudin-4. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Claudin 4,uniProtId:O14493-1,ncbiNodeId:9606,antigenRange:209,antigenLength:209,antigenImageFileName:32-9400_Claudin_4_O14493-1_House_mouse.svg,antigenImageFileNamePDP:32-9400_Claudin_4_O14493-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• Alexa Fluor 594
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533096

Product Specific Information

Reactivity has been confirmed with human, rat ileum homogenates, and canine MDCK cell lysates by western blotting and immunofluorescence. Reactivity has also been confirmed with formalin-fixed, paraffin-embedded (FFPE) human normal colon and colon cancer tissues by immunohistochemistry.

For best results in immunohistochemistry with formalin-fixed, paraffin-embedded (FFPE) tissues, heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining.

This antibody reacts specifically with the ~ 22 kDa Claudin-4 protein and does not cross-react with the protein at 55 kDa.

Target Information

Claudin-4 belongs to the 20-member family of claudins, transmembrane cellular adhesion proteins localized at epithelial and endothelial tight junctions. Claudins demonstrate distinct, tissue-specific expression patterns, and are essential components of tight junction charge-specific channels that regulate paracellular ion flux. Claudin-4 is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. The overexpression of Claudin-4 has been shown to decrease paracellular electrical conductance due to a selective decrease in Na+ permeability, with no significant decrease in Cl- permeability. In ovarian carcinoma cells, Claudin-3 and Claudin-4 exhibit upregulation at both mRNA and protein levels. Claudin-4 is also overexpressed in pancreatic cancer tissues and cell lines, and in other epithelial cancers. The Claudin-4 gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.