Thermo Fisher Scientific p38 MAPK Monoclonal Antibody (p38-3F11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0 µg/mL

View 11 publications 11 publications

Immunocytochemistry (ICC/IF)

5-20 µg/mL

View 1 publication 1 publication

ELISA (ELISA)

0.1-1.0 µg/mL

-

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Rabbit, Rat

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

p38-3F11

Immunogen

Full-length recombinant mouse p38 protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:p38 MAPK,uniProtId:P47811-1,ncbiNodeId:10090,antigenRange:1-360,antigenLength:360,antigenImageFileName:33-1300_p38_MAPK_P47811-1_House_mouse.svg,antigenImageFileNamePDP:33-1300_p38_MAPK_P47811-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533100

Product Specific Information

33-1300 has been successfully used in immunofluorescence, and western blot with human recombinant proteins and whole cell lysates of hamster, mouse, and primate origins.

33-1300 specifically recognizes both alpha and beta isoforms of p38, with slight reactivity to p38-delta.

Target Information

The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.