Thermo Fisher Scientific Microphthalmia Transcription Factor (MITF) Recombinant Rabbit Monoclonal Antibody (MITF/2987R)
MITF 단백질을 표적으로 하는 Thermo Fisher Scientific의 재조합 토끼 단일클론 항체로, 인간 및 개 시료에 반응합니다. IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합하며, Protein A/G 정제된 액상 형태로 제공됩니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | MITF/2987R |
| Immunogen | Recombinant full-length human MiTF protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Does not react with Mouse or Rat
Target Information
Mi (Microphthalmia Transcription Factor, MITF) is a basic helix-loop-helix-leucine zipper (b-HLH-ZIP) transcription factor involved in pigmentation, mast cell function, and bone development.
Mutations in Mi cause Waardenburg Syndrome type II in humans. In mice, loss of Mi leads to absence of pigmented cells in skin, eyes, and inner ear, as well as osteopetrosis and defects in natural killer and mast cells.
Two isoforms exist, differing by 66 amino acids at the N-terminus.
- Shorter forms: expressed in melanocytes, observed as two bands at 52 kDa and 56 kDa.
- Longer forms: expressed in osteoclasts, B16 melanoma cells, mast cells, and heart, appearing as clusters at 60–70 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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