Thermo Fisher Scientific ATG12 Polyclonal Antibody
ATG12 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, ICC/IF, IP에 사용 가능. Mouse 시료 반응성 검증됨. 항원 친화 크로마토그래피로 정제되었으며, 보존제 무첨가 액상 형태. 자가포식 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues near the amino terminus of mouse Atg12 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 29 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 100 µg/mL BSA, 50% glycerol, 0.15M NaCl |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10985246 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
ATG12 is a member of the autophagy protein family that forms a conjugate with ATG5. This conjugate exhibits ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. It also associates with innate immune response proteins such as RIG-I and VISA (IPS-1), inhibiting type I interferon production and allowing viral replication in host cells.
ATG12 interacts with ATG10 in human embryonic kidney cells in the presence of ATG7, and at least two isoforms of ATG12 are known.
Autophagy, a process of bulk degradation of cellular proteins through the autophagosomic-lysosomal pathway, is essential for normal growth control and may be defective in tumor cells. It preserves cellular nutrients under starvation and regulates cytosolic component turnover. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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