Thermo Fisher Scientific HLA-A3 Monoclonal Antibody (GAP.A3), NovaFluor Blue 510, eBioscience

Thermo Fisher Scientific HLA-A3 Monoclonal Antibody (GAP.A3), NovaFluor Blue 510, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats

• APC
• FITC
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• Super Bright 436
• Super Bright 600
• Request custom conjugation available

Product Specific Information

The monoclonal antibody GAP.A3 reacts with the human major histocompatibility complex (MHC) class I molecule HLA-A3, a member of the HLA-A family.
HLA-A3 exists as two subtypes, HLA-A3.1 and HLA-A3.2, differing at amino acid positions 152 and 156.
MHC class I molecules are expressed on all nucleated cells and present intracellular peptides on the cell surface, recognized by cytotoxic T lymphocytes.
HLA-A3 is expressed in 15–25% of cancer patients and binds to tumor-associated antigen hTERT.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated using normal human peripheral blood cells at 5 µL (0.6 µg) per test.
A test is defined as staining a cell sample in a final volume of 100 µL (10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures to minimize spillover and spread impacts.

Excitation: 496 nm
Emission: 511 nm
Laser: 488 nm (Blue)

Target Information

HLA-A belongs to the HLA class I heavy chain paralogues.
It is a heterodimer of a heavy chain and beta-2 microglobulin.
Class I molecules present peptides from the endoplasmic reticulum lumen and are expressed in nearly all cells.
The heavy chain (~45 kDa) gene has 8 exons:

• Exon 1: leader peptide
• Exons 2–3: α1 and α2 domains (peptide binding)
• Exon 4: α3 domain
• Exon 5: transmembrane region
• Exons 6–7: cytoplasmic tail

Polymorphisms in exons 2–3 determine peptide binding specificity.
Typing of these polymorphisms is routine in bone marrow and kidney transplantation.
Hundreds of HLA-A alleles have been described.

HLA and MHC antibodies are crucial in Immunopeptidomics for neoantigen identification and characterization via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지