Thermo Fisher Scientific HLA-A3 Monoclonal Antibody (GAP.A3), NovaFluor Blue 610-70S, eBioscience

Thermo Fisher Scientific HLA-A3 Monoclonal Antibody (GAP.A3), NovaFluor Blue 610-70S, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution

• 5 µL (0.6 µg)/test

Product Specifications

Additional Formats Available

• APC
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• Super Bright 436
• Super Bright 600
• Custom conjugation available

Product Specific Information

The monoclonal antibody GAP.A3 reacts with the human major histocompatibility complex (MHC) class I molecule HLA-A3, a member of the HLA-A family. HLA-A3 exists as two subtypes, HLA-A3.1 and HLA-A3.2, differing at amino acid positions 152 and 156. MHC class I molecules are expressed on all nucleated cells and present intracellular peptides to cytotoxic T lymphocytes. HLA-A3 is expressed by 15–25% of cancer patients and binds tumor-associated antigen hTERT.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: For use in flow cytometric analysis.
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells. Use at 5 µL (0.6 µg) per test (100 µL final volume, 10^5–10^8 cells/test).

Compatibility Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability detection.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01):

• Required for optimal NovaFluor staining.
• Add 5 µL per sample before combining with cells.
• For single-color controls, use 5 µL per 100 µL of cell sample (10^3–10^8 cells).

NovaFluor Dyes:

• Based on Phiton™ technology utilizing novel nucleic acid dye structures for engineered fluorescent signatures with reduced spillover and spread.

Excitation / Emission / Laser:

• Excitation: 509 nm
• Emission: 614 nm
• Laser: 488 nm (Blue)

Target Information

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (β2-microglobulin). The heavy chain (~45 kDa) is membrane-anchored and encoded by 8 exons:

• Exon 1: leader peptide
• Exons 2–3: α1 and α2 domains (peptide binding)
• Exon 4: α3 domain
• Exon 5: transmembrane region
• Exons 6–7: cytoplasmic tail

Polymorphisms in exons 2 and 3 define peptide binding specificity. Typing for these polymorphisms is routine in bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.

HLA and MHC antibodies are key tools in Immunopeptidomics, aiding neoantigen identification and characterization via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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