Thermo Fisher Scientific IGF1R alpha Monoclonal Antibody (1H7)
IGF1R alpha 단백질을 인식하는 Mouse monoclonal antibody(1H7)로, Western blot, IHC, Flow cytometry, ChIP 등 다양한 응용에 적합. Human 반응성, preservative-free 포뮬레이션이며, 저 endotoxin 수준(<0.01 EU/µg)을 유지. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | Assay-dependent | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:40 | Requires protein digestion (trypsin or pronase) |
| Flow Cytometry (Flow) | 1:10 | Use 10 µL per 1×10⁶ cells in 100 µL |
| ChIP assay (ChIP) | – | |
| Functional Assay | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1H7 |
| Immunogen | Proprietary immunogen |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | –20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_927947 |
Additional Formats
Product Specific Information
Clone 1H7 recognizes an epitope in the alpha subunit of CD221 (IGF1R), demonstrated by Western blotting. It partially blocks IGF-I and IGF-II binding to CD221.
For IHC applications, protein digestion (trypsin or pronase) is required on paraffin sections. Suggested positive controls include pancreas or placenta.
For flow cytometry, use 10 µL of the working dilution to label 1×10⁶ cells in 100 µL.
This antibody is low-endotoxin (<0.01 EU/µg) and preservative-free.
Target Information
IGF1R (Insulin-like Growth Factor 1 Receptor) is a receptor tyrosine kinase activated by IGF-1 and IGF-2, implicated in various cancers.
The protein is a tetramer with two alpha and two beta subunits. The alpha subunits bind IGF-1 with higher affinity than IGF-2, triggering autophosphorylation at residues 1134, 1135, and 1136.
Activated IGF1R phosphorylates downstream substrates, leading to activation of RAS, RAF, ERK, p38, and JNK pathways, which promote cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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