Thermo Fisher Scientific CD200 Monoclonal Antibody (OX104), NovaFluor Red 685, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.4 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant Violet 711
• eFluor 450
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Yellow 610
• NovaFluor Yellow 700
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody OX104 recognizes human CD200 (OX2), a member of the Ig superfamily with two Ig domains, a transmembrane, and a cytoplasmic domain. CD200 is expressed on resting and activated B cells, subsets of T cells, keratinocytes, nerve cells, follicular dendritic cells, and ovarian cells. Interaction with CD200R modulates immune responses by attenuating macrophage and mast cell activation.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on normal human peripheral blood cells. Recommended ≤0.06 µg per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize titration for best results.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  • Add 5 µL per sample or per 100 µL of cell sample (10³–10⁸ cells).
  • Add buffer to antibody cocktails before combining with cells.

NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered for optimized fluorescence with minimal spillover and spread.

Excitation: 637 nm
Emission: 685 nm
Laser: 633–640 nm (Red)

Target Information

CD200 is a type-1 membrane glycoprotein with two immunoglobulin domains, part of the immunoglobulin superfamily. It interacts with CD200R on myeloid cells to inhibit macrophage activation. Homologues exist in herpesviruses and poxviruses. CD200 regulates myeloid cell activity and provides inhibitory signals in diverse tissues. Dysregulation is associated with Mantle Cell Lymphoma and Primary Hypertrophic Osteoarthropathy.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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