Thermo Fisher Scientific NOV Polyclonal Antibody, PeproTech

Thermo Fisher Scientific NOV Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MQVAATQRCP PQCPGRCPAT PPTCAPGVRA VLDGCSCCLV CARQRGESCS DLEPCDESSG LYCDRSADPS NQTGICTAVE GDNCVFDGVI YRSGEKFQPS CKFQCTCRDG QIGCVPRCQL DVLLPEPNCP APRKVEVPGE CCEKWICGPD EEDSLGGLTL AAYRPEATLG VEVSDSSVNC IEQTTEWTAC SKSCGMGFST RVTNRNRQCE MLKQTRLCMV RPCEQEPEQP TDKKGKKCLR TKKSLKAIHL QFKNCTSLHT YKPRFCGVCS DGRCCTPHNT KTIQAEFQCS PGQIVKKPVM VIGTCTCHTN CPKNNEAFLQ ELELKTTRGK M

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human NOV. Anti-Human NOV-specific antibody was purified by affinity chromatography employing an immobilized Human NOV matrix.

Sandwich ELISA:
To detect Human NOV by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human NOV (500-P257Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human NOV.

Western Blot:
To detect Human NOV by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human NOV is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

NOV is a member of the CCN family of secreted, cysteine-rich regulatory proteins. The full-length NOV protein contains four structural domains that confer distinct, and sometimes opposing, biological activities. Elevated expression of NOV is associated with certain tumors, including Wilm’s tumor and most nephroblastomas. However, in other tumor types and certain cancer cell lines, increased tumorigenicity and proliferation is correlated with decreased NOV expression. Additionally, NOV induces cell adhesion and migration by signaling through specific cell-surface integrins and by binding to heparin sulfate proteoglycans and fibulin 1C. NOV has also been reported to exert proangiogenic activities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.