Thermo Fisher Scientific NOV Polyclonal Antibody, PeproTech
Human NOV 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot 및 ELISA 분석에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, Lyophilized 형태로 제공됩니다. 암 관련 연구 및 세포 신호전달 연구에 활용 가능합니다.
- 카탈로그번호
- 500-P257-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific NOV Polyclonal Antibody, PeproTech
Thermo Fisher Scientific NOV Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human NOV |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient |
| RRID | AB_2930104 |
Product Specific Information
AA Sequence of recombinant protein:
MQVAATQRCP PQCPGRCPAT PPTCAPGVRA VLDGCSCCLV CARQRGESCS DLEPCDESSG LYCDRSADPS NQTGICTAVE GDNCVFDGVI YRSGEKFQPS CKFQCTCRDG QIGCVPRCQL DVLLPEPNCP APRKVEVPGE CCEKWICGPD EEDSLGGLTL AAYRPEATLG VEVSDSSVNC IEQTTEWTAC SKSCGMGFST RVTNRNRQCE MLKQTRLCMV RPCEQEPEQP TDKKGKKCLR TKKSLKAIHL QFKNCTSLHT YKPRFCGVCS DGRCCTPHNT KTIQAEFQCS PGQIVKKPVM VIGTCTCHTN CPKNNEAFLQ ELELKTTRGK M
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human NOV. Anti-Human NOV-specific antibody was purified by affinity chromatography employing an immobilized Human NOV matrix.
Sandwich ELISA:
To detect Human NOV by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human NOV (500-P257Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human NOV.
Western Blot:
To detect Human NOV by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human NOV is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
NOV is a member of the CCN family of secreted, cysteine-rich regulatory proteins. The full-length NOV protein contains four structural domains that confer distinct, and sometimes opposing, biological activities. Elevated expression of NOV is associated with certain tumors, including Wilm’s tumor and most nephroblastomas. However, in other tumor types and certain cancer cell lines, increased tumorigenicity and proliferation is correlated with decreased NOV expression. Additionally, NOV induces cell adhesion and migration by signaling through specific cell-surface integrins and by binding to heparin sulfate proteoglycans and fibulin 1C. NOV has also been reported to exert proangiogenic activities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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