Thermo Fisher Scientific Ep-CAM/CD326 Recombinant Rabbit Monoclonal Antibody (EGP40, 2041R)
Ep-CAM/CD326 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot 및 IHC(파라핀 절편)에 적합합니다. HEK293 세포에서 발현된 IgG 형식의 항체이며, 단백질 A로 정제되었습니다. 인간, 개, 고양이 시료에 반응하며 4°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Cat, Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | EGP40, 2041R |
| Immunogen | Recombinant human EpCAM fragment from extracellular domain (around aa100–224) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- For formalin-fixed tissues: Heat tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes before staining.
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent cell adhesion molecule influencing cell cycle, proliferation, and metabolism by inducing c-myc and cyclins A/E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression correlates with enhanced epithelial proliferation and is observed in many human carcinomas, serving as a marker for epithelial lineage tumors.
Ep-CAM is localized on the basolateral surface of most simple epithelia and various carcinoma types, and helps distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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