Thermo Fisher Scientific Rat anti-Mouse IgG1 Secondary Antibody, eFluor 660, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugate Formats

• APC
• Biotin
• eFluor 450
• eFluor 570
• eFluor 615
• FITC
• HRP
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Custom conjugation available upon request

Product Specific Information

The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and is suitable as a secondary reagent in flow cytometry and microscopy. It does not recognize other mouse isotypes and shows no cross-reactivity with rat IgG1 or other rat isotypes.

Applications Reported: Flow cytometry, microscopy, immunohistochemistry, and immunocytochemistry.

Applications Tested:

• Flow cytometry: ≤ 0.25 µg/test (defined for 100 µL final volume, 10⁵–10⁸ cells/test).
• Immunocytochemistry / Immunohistochemistry: ≤ 10 µg/mL.
  It is recommended to titrate the antibody for optimal performance.

Excitation: 633–647 nm
Emission: 668 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are used for detection, sorting, or purification of mouse antibodies. Secondary antibodies provide versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. These reagents are generated by immunizing host animals with pooled mouse immunoglobulins and purified via immunoaffinity chromatography or conjugation to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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