Thermo Fisher Scientific CNOT4 Monoclonal Antibody (OTI7D7), TrueMAB

Applications

• Western Blot (WB)
  Tested Dilution: 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

CNOT4 is a 575 amino acid protein with E3 ubiquitin ligase activity, involved in protein modification and ubiquitination.
It contains:

• A C4C4-type RING zinc finger domain
• An RNA recognition motif
• A bipartite nuclear localization signal

CNOT4 is a component of the CCR4–NOT (CAF1) core complex, which regulates transcription of multiple genes both positively and negatively.
It interacts with CNOT1 via its C-terminus and with E2 ubiquitin ligases via its RING domain.
Reports indicate that CNOT4 is phosphorylated in response to DNA damage at consensus sites recognized by ATM and ATR.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.