Thermo Fisher Scientific UBE2E3 Monoclonal Antibody (OTI3E5), TrueMAB

Applications and Tested Dilutions

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates.

1. The first step requires ATP-dependent activation of the Ub C-terminus and assembly of multi-Ub chains by the E1 enzyme.
2. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to form an intermediate Ub–E2 complex.
3. The Ub-ligase (E3) catalyzes the transfer of Ub from E2 to the target protein substrate.

UBE2E3 (also known as UBCH9) catalyzes the covalent attachment of ubiquitin to other proteins and is involved in the regulation of transepithelial sodium transport in renal cells. It may also play a role in cell growth arrest. The UBE2E3 protein shuttles between the cytoplasm and nucleus in an IPO11-dependent manner and is ubiquitously expressed at low levels, with high expression in skeletal muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.