Thermo Fisher Scientific Phospho-PDPK1 (Ser241) Recombinant Rabbit Monoclonal Antibody (PDK1S241-F7), FITC

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL/1x10^6 cells

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

PDK1S241-F7

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Ser241 of human phospho PDPK1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PDPK1,uniProtId:O15530-1,ncbiNodeId:9606,antigenRange:241,antigenLength:556,antigenImageFileName:MA5-37090_PDPK1_O15530-1_Rabbit.svg,antigenImageFileNamePDP:MA5-37090_PDPK1_O15530-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

FITC FITC FITC

View additional formats

• Unconjugated
• APC
• PE
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Purification

Protein A/G

Storage buffer

PBS with 0.20% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2897024

Target Information

PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDPK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDPK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.