Thermo Fisher Scientific Phospho-Lck (Tyr505) Recombinant Rabbit Monoclonal Antibody (LckY505-A3)

Applications

Western Blot (WB)

• Assay-dependent

Flow Cytometry (Flow)

• 1–1,000 ng/mL

Product Specifications

Additional Formats

• APC
• FITC
• PE
• Request custom conjugation

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:

• Improved specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to the rabbit immune repertoire

Target Information

LCK is a member of the Src-family tyrosine kinases essential for T-cell receptor (TCR) signaling. Upon TCR activation, LCK phosphorylates ITAM motifs in zeta subunits, enabling binding of ZAP70, which is subsequently phosphorylated and activated.
LCK is primarily localized to the plasma membrane but also found in the Golgi apparatus, contributing to Raf activation under weak TCR stimulation.
It contains N-terminal myristylation and palmitylation sites, a PTK domain, and SH2/SH3 domains mediating protein-protein interactions.
LCK is involved in regulating apoptosis and is associated with Immunodeficiency 22 and CD45 deficiency. Alternative splice variants exist encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.