Thermo Fisher Scientific Dicer Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

Flow Cytometry (Flow)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein within human aa 701-1100/1922. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Dicer,uniProtId:Q9UPY3-1,ncbiNodeId:9606,antigenRange:701-1100,antigenLength:1922,antigenImageFileName:PA5-144679_Dicer_Q9UPY3-1_Rabbit.svg,antigenImageFileNamePDP:PA5-144679_Dicer_Q9UPY3-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3093520

Product Specific Information

Positive control: MCF-7 cell lysate, 293 cell lysate, Daudi cell lysate, MCF-7, C2C12.

Predicted band size: 218 kDa

Subcellular Location: Cytoplasm, perinuclear region.

Target Information

Dicer is a double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. It cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3` overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a means to specifically regulate the expression of target genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.