Thermo Fisher Scientific HP1 alpha Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

• Immunogen sequence:
  MGKKTKRTAD SSSSEDEEEY VVEKVLDRRV VKGQVEYLLK WKGFSEEHNT WEPEKNLDCP ELISEFMKKY KKMKEGENNK PREKSESNKR KSNFSNSADD IKSKKKREQS NDIARGFERG LEPEKIIGAT DSCGDLMFLM KWKDTDEADL VLAKEANVKC PQIVIAFYEE RLTWHAYPED AENKEKETAK S
• Positive Samples: HeLa, SH-SY5Y, A549, MCF-7
• Cellular Location: Chromosome, Nucleus, Centromere

Target Information

Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. Mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of adaptor molecules implicated in gene silencing and chromatin structure. High-affinity in vitro recognition of methylated histone H3 by HP1 requires a functional chromodomain, which interacts specifically with the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-mutant fibroblasts but restored after reintroduction of catalytically active SUV39H1 HMTase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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