Thermo Fisher Scientific ASAH2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human ASAH2 (NP_0011374461) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ASAH2,uniProtId:Q9NR71-1,ncbiNodeId:9606,antigenRange:80-340,antigenLength:780,antigenImageFileName:PA5-88650_ASAH2_Q9NR71-1_Rabbit.svg,antigenImageFileNamePDP:PA5-88650_ASAH2_Q9NR71-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.7 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2805052

Product Specific Information

Immunogen sequence: TATQTSPVPL TPESPLFQNF SGYHIGVGRA DCTGQVADIN LMGYGKSGQN AQGILTRLYS RAFIMAEPDG SNRTVFVSID IGMVSQRLRL EVLNRLQSKY GSLYRRDNVI LSGTHTHSGP AGYFQYTVFV IASEGFSNQT FQHMVTGILK SIDIAHTNMK PGKIFINKGN VDGVQINRSP YSYLQNPQSE RARYSSNTDK EMIVLKMVDL NGDDLGLISW FAIHPVSMNN SNHLVNSDNV GYASYLLEQE KNKGYLPGQG P; Positive Samples: 22Rv1, Mouse kidney, Rat kidney; Cellular Location: Cell membrane, Single-pass type II membrane protein

Target Information

Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH2 is a neutral ceramidase and key regulator of sphingolipid signaling metabolites at the cell surface, catalyzing the hydrolysis of the N-acyl linkage of ceramide at an optimal pH of 6.5-8.5. ASAH2 is a type II integral membrane protein that can be cleaved to yield a soluble secreted protein and acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger. ASAH2 is ubiquitously expressed primarily expressed with higher levels in the intestine, kidney, skeletal muscle and heart. Recent studies indicate that ASAH2 encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.